Multicenter Collaborative Study to Optimize Mass Spectrometry Workflows of Clinical Specimens.

ABSTRACT: The foundation for integrating mass spectrometry (MS)-based proteomics into systems medicine is the development of standardized start-to-finish and fit-for-purpose workflows for clinical specimens. An essential step in this pursuit is to highlight the common ground in a diverse landscape of different sample preparation techniques and liquid chromatography-mass spectrometry (LC-MS) setups. With the aim to benchmark and improve the current best practices among the proteomics MS laboratories of the CLINSPECT-M consortium, we performed two consecutive round-robin studies with full freedom to operate in terms of sample preparation and MS measurements. The six study partners were provided with two clinically relevant sample matrices: plasma and cerebrospinal fluid (CSF). In the first round, each

SUBMITTER: Kardell O 

PROVIDER: S-EPMC10775142 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature