Proteomic profile of tepary bean seed storage proteins in germination with low water potential.

ABSTRACT:

Background

Tepary bean (Phaseolus acutifolius A. Gray) is one of the five species domesticated from the genus Phaseolus with genetic resistance to biotic and abiotic stress. To understand the mechanisms underlying drought responses in seed storage proteins germinated on water and polyethylene glycol (PEG-6000) at -0.49 MPa, we used a proteomics approach to identify potential molecular target proteins associated with the low water potential stress response.

Methods

Storage proteins from cotyledons of Tepary bean seeds germinated at 24, 48 and 72 h on water and PEG-6000 at -0.49 MPa were analyzed by one-dimensional electrophoresis (DE) with 2-DE analysis and shotgun mass spectrometry. Using computational database searching and bioinformatics analyses, we performed Gene Ontolog

SUBMITTER: Padilla-Chacon D 

PROVIDER: S-EPMC10775562 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature