Matrigel Tunes H9 Stem Cell-Derived Human Cerebral Organoid Development.

ABSTRACT: Human cerebral organoids are readily generated from human embryonic stem cells and human induced pluripotent stem cells and are useful in studying human neurodevelopment. Recent work with human cerebral organoids have explored the creation of different brain regions and the impacts of soluble and mechanical cues. Matrigel is a gelatinous, heterogenous mixture of extracellular matrix proteins, morphogens, and growth factors secreted by Engelbreth-Holm-Swarm mouse sarcoma cells. It is a core component of almost all cerebral organoid protocols, generally supporting neuroepithelial budding and tissue polarization; yet, its roles and effects beyond its general requirement in organoid protocols are not well understood, and its mode of delivery is variable, including the embedding of organoids wi

SUBMITTER: Estridge RC 

PROVIDER: S-EPMC10776236 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature