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Accurate prediction of protein-nucleic acid complexes using RoseTTAFoldNA.

ABSTRACT: Protein-RNA and protein-DNA complexes play critical roles in biology. Despite considerable recent advances in protein structure prediction, the prediction of the structures of protein-nucleic acid complexes without homology to known complexes is a largely unsolved problem. Here we extend the RoseTTAFold machine learning protein-structure-prediction approach to additionally predict nucleic acid and protein-nucleic acid complexes. We develop a single trained network, RoseTTAFoldNA, that rapidly produces three-dimensional structure models with confidence estimates for protein-DNA and protein-RNA complexes. Here we show that confident predictions have considerably higher accuracy than current state-of-the-art methods. RoseTTAFoldNA should be broadly useful for modeling the structure of naturally occurring protein-nucleic acid complexes, and for designing sequence-specific RNA and DNA-binding proteins.

SUBMITTER: Baek M

PROVIDER: S-EPMC10776382 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature

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Accurate prediction of protein-nucleic acid complexes using RoseTTAFoldNA.

Baek Minkyung M McHugh Ryan R Anishchenko Ivan I Jiang Hanlun H Baker David D DiMaio Frank F

Nature methods 20231123 1

Protein-RNA and protein-DNA complexes play critical roles in biology. Despite considerable recent advances in protein structure prediction, the prediction of the structures of protein-nucleic acid complexes without homology to known complexes is a largely unsolved problem. Here we extend the RoseTTAFold machine learning protein-structure-prediction approach to additionally predict nucleic acid and protein-nucleic acid complexes. We develop a single trained network, RoseTTAFoldNA, that rapidly pr ...[more]

PMID: 37996753

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