Dataset Information

11-deoxycortisol positively correlates with T cell immune traits in physiological conditions.

ABSTRACT:

Background

Endogenous steroid hormones have significant effects on inflammatory and immune processes, but the immunological activities of steroidogenesis precursors remain largely unexplored.

Methods

We conducted a systematic approach to examine the association between steroid hormones profile and immune traits in a cohort of 534 healthy volunteers. Serum concentrations of steroid hormones and their precursors (cortisol, progesterone, testosterone, androstenedione, 11-deoxycortisol and 17-OH progesterone) were determined by liquid chromatography-tandem mass spectrometry. Immune traits were evaluated by quantifying cellular composition of the circulating immune system and ex vivo cytokine responses elicited by major human pathogens and microbial ligands. An independent cohort of 321 individuals was used for validation, followed by in vitro validation experiments.

Findings

We observed a positive association between 11-deoxycortisol and lymphoid cellular subsets numbers and function (especially IL-17 response). The association with lymphoid cellularity was validated in an independent validation cohort. In vitro experiments showed that, as compared to androstenedione and 17-OH progesterone, 11-deoxycortisol promoted T cell proliferation and Candida-induced Th17 polarization at physiologically relevant concentrations. Functionally, 11-deoxycortisol-treated T cells displayed a more activated phenotype (PD-L1^high CD25^high CD62L^low CD127^low) in response to CD3/CD28 co-stimulation, and downregulated expression of T-bet nuclear transcription factor.

Interpretation

Our findings suggest a positive association between 11-deoxycortisol and T-cell function under physiological conditions. Further investigation is needed to explore the potential mechanisms and clinical implications.

Funding

Found in acknowledgements.

SUBMITTER: Peng C

PROVIDER: S-EPMC10776925 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature

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Publications

11-deoxycortisol positively correlates with T cell immune traits in physiological conditions.

Peng Chunying C Jiang Xun X Jaeger Martin M van Houten Pepijn P van Herwaarden Antonius E AE Koeken Valerie A C M VACM Moorlag Simone J C F M SJCFM Mourits Vera P VP Lemmers Heidi H Dijkstra Helga H Koenen Hans J P M HJPM Joosten Irma I van Cranenbroek Bram B Li Yang Y Joosten Leo A B LAB Netea Mihai G MG Netea-Maier Romana T RT Xu Cheng-Jian CJ

EBioMedicine 20231221

<h4>Background</h4>Endogenous steroid hormones have significant effects on inflammatory and immune processes, but the immunological activities of steroidogenesis precursors remain largely unexplored.<h4>Methods</h4>We conducted a systematic approach to examine the association between steroid hormones profile and immune traits in a cohort of 534 healthy volunteers. Serum concentrations of steroid hormones and their precursors (cortisol, progesterone, testosterone, androstenedione, 11-deoxycortiso ...[more]

PMID: 38134621

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Dataset Information

11-deoxycortisol positively correlates with T cell immune traits in physiological conditions.

Background

Methods

Findings

Interpretation

Funding

Publications

11-deoxycortisol positively correlates with T cell immune traits in physiological conditions.

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