Arp2/3 complex and the pentose phosphate pathway regulate late phases of neutrophil swarming.

ABSTRACT: Neutrophil swarming is an essential process of the neutrophil response to many pathological conditions. Resultant neutrophil accumulations are hallmarks of acute tissue inflammation and infection, but little is known about their dynamic regulation. Technical limitations to spatiotemporally resolve individual cells in dense neutrophil clusters and manipulate these clusters in situ have hampered recent progress. We here adapted an in vitro swarming-on-a-chip platform for the use with confocal laser-scanning microscopy to unravel the complexity of single-cell responses during neutrophil crowding. Confocal sectioning allowed the live visualization of subcellular components, including mitochondria, cell membranes, cortical actin, and phagocytic cups, inside neutrophil clusters. Ba

SUBMITTER: Glaser KM 

PROVIDER: S-EPMC10777075 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature