Skeletal Muscle Spheroids as Building Blocks for Engineered Muscle Tissue.

ABSTRACT: Spheroids exhibit enhanced cell-cell interactions that facilitate improved survival and mimic the physiological cellular environment in vivo. Cell spheroids have been successfully used as building blocks for engineered tissues, yet the viability of this approach with skeletal muscle spheroids is poorly understood, particularly when incorporated into three-dimensional (3D) constructs. Bioprinting is a promising strategy to recapitulate the hierarchical organization of native tissue that is fundamental to its function. However, the influence of bioprinting on skeletal muscle cell spheroids and their function are yet to be interrogated. Using C2C12 mouse myoblasts and primary bovine muscle stem cells (MuSCs), we characterized spheroid formation as a function of duration and cell seedin

SUBMITTER: Johnson N 

PROVIDER: S-EPMC10777344 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature