De Novo Single-Stranded RNA-Binding Peptides Discovered by Codon-Restricted mRNA Display.

ABSTRACT: RNA-binding proteins participate in diverse cellular processes, including DNA repair, post-transcriptional modification, and cancer progression through their interactions with RNAs, making them attractive for biotechnological applications. While nature provides an array of naturally occurring RNA-binding proteins, developing de novo RNA-binding peptides remains challenging. In particular, tailoring peptides to target single-stranded RNA with low complexity is difficult due to the inherent structural flexibility of RNA molecules. Here, we developed a codon-restricted mRNA display and identified multiple de novo peptides from a peptide library that bind to poly(C) and poly(A) RNA with K_Ds ranging from micromolar to submicromolar concentrations. One of the newl

SUBMITTER: Nishikawa S 

PROVIDER: S-EPMC10777347 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature