Identification of CD8⁺ T-cell exhaustion signatures for prognosis in HBV-related hepatocellular carcinoma patients by integrated analysis of single-cell and bulk RNA-sequencing.

ABSTRACT:

Background

HBV infection is the leading risk factor for HCC. HBV infection has been confirmed to be associated with the exhaustion status of CD8⁺ T cells and immunotherapeutic efficacy in HCC. In this study, we aimed to investigate the prognostic value of the CD8⁺ T-cell exhaustion signature and immunotherapy response in patients with HBV-related HCC.

Methods

We identified different clusters of HBV-related HCC cells by single-cell RNA sequencing (scRNA-seq) and identified CD8⁺ T-cell exhaustion-related genes (TERGs) by pseudotime analysis. We conducted differential expression analysis and LASSO Cox regression to detect genes and construct a CD8⁺ T-cell exhaustion index (TEI). We next combined the TEI with other clinicopathological

SUBMITTER: Li J 

PROVIDER: S-EPMC10777580 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature