Tissue lipidomic profiling supports a mechanistic role of the prostaglandin E2 pathway for albuminuria development in glomerular hyperfiltration.

ABSTRACT: Background: Glomerular hyperfiltration (GH) is an important mechanism in the development of albuminuria in hypertension. The Munich Wistar Frömter (MWF) rat is a non-diabetic model of chronic kidney disease (CKD) with GH due to inherited low nephron number resulting in spontaneous albuminuria and podocyte injury. In MWF rats, we identified prostaglandin (PG) E₂ (PGE₂) signaling as a potential causative mechanism of albuminuria in GH. Method: For evaluation of the renal PGE₂ metabolic pathway, time-course lipidomic analysis of PGE₂ and its downstream metabolites 15-keto-PGE₂ and 13-14-dihydro-15-keto-PGE₂ was conducted in urine, plasma and kidney tissues of MWF rats and albuminuria-resistant spontaneously hypert

SUBMITTER: Kaiser-Graf D 

PROVIDER: S-EPMC10777844 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature