VDR and PDIA3 Are Essential for Activation of Calcium Signaling and Membrane Response to 1,25(OH)₂D₃ in Squamous Cell Carcinoma Cells.

ABSTRACT: The genomic activity of 1,25(OH)₂D₃ is mediated by vitamin D receptor (VDR), whilst non-genomic is associated with protein disulfide isomerase family A member 3 (PDIA3). Interestingly, our recent studies documented that PDIA3 is also involved, directly or indirectly, in the modulation of genomic response to 1,25(OH)₂D₃. Moreover, PDIA3 was also shown to regulate cellular bioenergetics, possibly through the modulation of STAT signaling. Here, the role of VDR and PDIA3 proteins in membrane response to 1,25(OH)₂D₃ and calcium signaling was investigated in squamous cell carcinoma A431 cell line with or without the deletion of VDR and PDIA3 genes. Calcium influx was assayed by Fura-2AM or Fluo-4AM, while calcium-reg

SUBMITTER: Nowak JI 

PROVIDER: S-EPMC10778127 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature