Characterization of Extracellular Vesicles from Human Saliva: Effects of Age and Isolation Techniques.

ABSTRACT: Salivary extracellular vesicles (EVs) represent an attractive source of biomarkers due to the accessibility of saliva and its non-invasive sampling methods. However, the lack of comparative studies assessing the efficacy of different EV isolation techniques hampers the use of salivary EVs in clinical settings. Moreover, the effects of age on salivary EVs are largely unknown, hindering the identification of salivary EV-associated biomarkers across the lifespan. To address these questions, we compared salivary EV concentration, size mode, protein concentration, and purity using eight EV isolation techniques before and after magnetic bead immunocapture with antibodies against CD9, CD63, and CD81. The effects of age on salivary EVs obtained with each isolation technique were further investigat

SUBMITTER: Reseco L 

PROVIDER: S-EPMC10778510 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature