Phosphoramidate Azole Oligonucleotides for Single Nucleotide Polymorphism Detection by PCR.

ABSTRACT: Detection of the Kirsten rat sarcoma gene (KRAS) mutational status is an important factor for the treatment of various malignancies. The most common KRAS-activating mutations are caused by single-nucleotide mutations, which are usually determined by using PCR, using allele-specific DNA primers. Oligonucleotide primers with uncharged or partially charged internucleotide phosphate modification have proved their ability to increase the sensitivity and specificity of various single nucleotide mutation detection. To enhance the specificity of single nucleotide mutation detection, the novel oligonucleotides with four types of uncharged and partially charged internucleotide phosphates modification, phosphoramide benzoazole (PABA) oligonucleotides (PABAO), was used to prove the conce

SUBMITTER: Chubarov AS 

PROVIDER: S-EPMC10778797 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature