C11-hydroxy and C11-oxo C₁₉ and C₂₁ Steroids: Pre-Receptor Regulation and Interaction with Androgen and Progesterone Steroid Receptors.

ABSTRACT: C11-oxy C₁₉ and C11-oxy C₂₁ steroids have been identified as novel steroids but their function remains unclear. This study aimed to investigate the pre-receptor regulation of C11-oxy steroids by 11β-hydroxysteroid dehydrogenase (11βHSD) interconversion and potential agonist and antagonist activity associated with the androgen (AR) and progesterone receptors (PRA and PRB). Steroid conversions were investigated in transiently transfected HEK293 cells expressing 11βHSD1 and 11βHSD2, while CV1 cells were utilised for agonist and antagonist assays. The conversion of C11-hydroxy steroids to C11-oxo steroids by 11βHSD2 occurred more readily than the reverse reaction catalysed by 11βHSD1, while the interconversion of C11-oxy C₁₉ steroids was more efficient than C11

SUBMITTER: Gent R 

PROVIDER: S-EPMC10778819 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature