Prophage Carriage and Genetic Diversity within Environmental Isolates of Clostridioides difficile.

ABSTRACT: Clostridioides difficile is an important human pathogen causing antibiotic-associated diarrhoea worldwide. Besides using antibiotics for treatment, the interest in bacteriophages as an alternative therapeutic option has increased. Prophage abundance and genetic diversity are well-documented in clinical strains, but the carriage of prophages in environmental strains of C. difficile has not yet been explored. Thus, the prevalence and genetic diversity of integrated prophages in the genomes of 166 environmental C. difficile isolates were identified. In addition, the clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems were determined in the genomes of prophage regions. Predicted prophages and CRISPR-Cas systems were identified by using the PHASTE

SUBMITTER: Blau K 

PROVIDER: S-EPMC10778935 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature