Elucidating Differences in Early-Stage Centrosome Amplification in Primary and Immortalized Mouse Cells.

ABSTRACT: The centrosome is involved in cytoplasmic microtubule organization during interphase and in mitotic spindle assembly during cell division. Centrosome amplification (abnormal proliferation of centrosome number) has been observed in several types of cancer and in precancerous conditions. Therefore, it is important to elucidate the mechanism of centrosome amplification in order to understand the early stage of carcinogenesis. Primary cells could be used to better understand the early stage of carcinogenesis rather than immortalized cells, which tend to have various genetic and epigenetic changes. Previously, we demonstrated that a poly(ADP-ribose) polymerase (PARP) inhibitor, 3-aminobenzamide (3AB), which is known to be nontoxic and nonmutagenic, could induce centrosome amplification and chro

SUBMITTER: Tanaka M 

PROVIDER: S-EPMC10778991 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature