An In Vitro Model for Acute Myeloid Leukemia Relapse Using the SORE6 Reporter.

ABSTRACT: Many patients diagnosed with acute myeloid leukemia (AML) relapse within two years of the initial remission. The biology of AML relapse is incompletely understood, although cancer stem-like (CSL) cells have been hypothesized to be important. To test this hypothesis, we employed SORE6, a reporter designed to detect the transcriptional activity of the embryonic stem cell proteins Oct4 and Sox2, to identify/purify CSL cells in two FLT3-mutated AML cell lines. Both cell lines contained ~10% of SORE6⁺ cells in the steady state. Compared to SORE6^- cells, SORE6⁺ cells exhibited more characteristics of CSL cells, with significantly higher chemoresistance and rates of spheroid formation. SORE6⁺ cells had substantially higher expression of Myc and FLT3 pro

SUBMITTER: Lai J 

PROVIDER: S-EPMC10779023 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature