Heterogeneity in Neutrophil Extracellular Traps from Healthy Human Subjects.

ABSTRACT: Neutrophil extracellular traps (NETs), a key component of early defense against microbial infection, are also associated with tissue injury. NET composition has been reported to vary with some disease states, but the composition and variability of NETs across many healthy subjects provide a critical comparison that has not been well investigated. We evaluated NETs from twelve healthy subjects of varying ages isolated from multiple blood draws over a three-and-one-half-year period to delineate the variability in extracellular DNA, protein, enzymatic activities, and susceptibility to protease inhibitors. We calculated correlations for NET constituents and loss of human bronchial epithelial barrier integrity, measured by transepithelial electrical resistance, after NET exposure. We found that

SUBMITTER: Collins MS 

PROVIDER: S-EPMC10779146 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature