Glutamate's Effects on the N-Methyl-D-Aspartate (NMDA) Receptor Ion Channel in Alzheimer's Disease Brain: Challenges for PET Radiotracer Development for Imaging the NMDA Ion Channel.

ABSTRACT: In an effort to further understand the challenges facing in vivo imaging probe development for the N-methyl-D-aspartate (NMDA) receptor ion channel, we have evaluated the effect of glutamate on the Alzheimer's disease (AD) brain. Human post-mortem AD brain slices of the frontal cortex and anterior cingulate were incubated with [³H]MK-801 and adjacent sections were tested for Aβ and Tau. The binding of [³H]MK-801 was measured in the absence and presence of glutamate and glycine. Increased [³H]MK-801 binding in AD brains was observed at baseline and in the presence of glutamate, indicating a significant increase (>100%) in glutamate-induced NMDA ion channel activity in AD brains compared to cognitively normal brains. The glycine effect was lower, suggesting a

SUBMITTER: Shah NM 

PROVIDER: S-EPMC10779680 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature