ULK2 Is a Key Pro-Autophagy Protein That Contributes to the High Chemoresistance and Disease Relapse in FLT3-Mutated Acute Myeloid Leukemia.

ABSTRACT: We recently demonstrated that a small subset of cells in FLT3-mutated acute myeloid leukemia (AML) cell lines exhibit SORE6 reporter activity and cancer stem-like features including chemoresistance. To study why SORE6⁺ cells are more chemoresistant than SORE6^- cells, we hypothesized that these cells carry higher autophagy, a mechanism linked to chemoresistance. We found that cytarabine (Ara-C) induced a substantially higher protein level of LC3B-II in SORE6⁺ compared to SORE6^- cells. Similar observations were made using a fluorescence signal-based autophagy assay. Furthermore, chloroquine (an autophagy inhibitor) sensitized SORE6⁺ but not SORE6^- cells to Ara-C. To decipher the molecular mechanisms underlying the high autopha

SUBMITTER: Lai J 

PROVIDER: S-EPMC10780038 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature