Emergence of replication timing during early mammalian development.

ABSTRACT: DNA replication enables genetic inheritance across the kingdoms of life. Replication occurs with a defined temporal order known as the replication timing (RT) programme, leading to organization of the genome into early- or late-replicating regions. RT is cell-type specific, is tightly linked to the three-dimensional nuclear organization of the genome^1,2 and is considered an epigenetic fingerprint³. In spite of its importance in maintaining the epigenome⁴, the developmental regulation of RT in mammals in vivo has not been explored. Here, using single-cell Repli-seq⁵, we generated genome-wide RT maps of mouse embryos from the zygote to the blastocyst stage. Our data show that RT is initially not well defined but becomes defined progressively from t

SUBMITTER: Nakatani T 

PROVIDER: S-EPMC10781638 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature