Selecting a preculture strategy for improving biomass and astaxanthin productivity of Chromochloris zofingiensis.

ABSTRACT: Chromochloris zofingiensis is a potential source of natural astaxanthin; however, its rapid growth and astaxanthin enrichment cannot be achieved simultaneously. This study established autotrophic, mixotrophic, and heterotrophic preculture patterns to assess their ameliorative effect on the C. zofingiensis heterotrophic growth state. In comparison, mixotrophic preculture (MP) exhibited the best improving effect on heterotrophic biomass concentration of C. zofingiensis (up to 121.5 g L^-1) in a 20 L fermenter, reaching the global leading level. The astaxanthin productivity achieved 111 mg L^-1 day^-1, 7.4-fold higher than the best record. The transcriptome and ¹³C tracer-based metabolic flux analysis were used for mechanism inquiry. The results reveal

SUBMITTER: Wang Y 

PROVIDER: S-EPMC10781847 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature