Identification of acidic stress-responsive genes and acid tolerance engineering in Synechococcus elongatus PCC 7942.

ABSTRACT: Cyanobacteria are excellent autotrophic photosynthetic chassis employed in synthetic biology, and previous studies have suggested that they have alkaline tolerance but low acid tolerance, significantly limiting their productivity as photosynthetic chassis and necessitating investigations into the acid stress resistance mechanism. In this study, differentially expressed genes were obtained by RNA sequencing-based comparative transcriptomic analysis under long-term acidic stress conditions and acidic shock treatment, in the model cyanobacterium Synechococcus elongatus PCC 7942. A pathway enrichment analysis revealed the upregulated and downregulated pathways during long-term acidic and shock stress treatment. The subsequent single gene knockout and phenotype analysis showed that under acidic

SUBMITTER: Zhang J 

PROVIDER: S-EPMC10781874 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature