Mosaic-PICASSO: accurate crosstalk removal for multiplex fluorescence imaging.

ABSTRACT:

Motivation

Ultra-multiplexed fluorescence imaging has revolutionized our understanding of biological systems, enabling the simultaneous visualization and quantification of multiple targets within biological specimens. A recent breakthrough in this field is PICASSO, a mutual-information-based technique capable of demixing up to 15 fluorophores without their spectra, thereby significantly simplifying the application of ultra-multiplexed fluorescence imaging. However, this study has identified a limitation of mutual information (MI)-based techniques. They do not differentiate between spatial colocalization and spectral mixing. Consequently, MI-based demixing may incorrectly interpret spatially co-localized targets as non-colocalized, leading to overcorrection.

Results

We found

SUBMITTER: Cang H 

PROVIDER: S-EPMC10781941 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature