Adaptive control of the E. coli-specific growth rate in fed-batch cultivation based on oxygen uptake rate.

ABSTRACT: In this study, an automatic control system is developed for the setpoint control of the cell biomass specific growth rate (SGR) in fed-batch cultivation processes. The feedback signal in the control system is obtained from the oxygen uptake rate (OUR) measurement-based SGR estimator. The OUR online measurements adapt the system controller to time-varying operating conditions. The developed approach of the PI controller adaptation is presented and discussed. The feasibility of the control system for tracking a desired biomass growth time profile is demonstrated with numerical simulations and fed-batch culture E.coli control experiments in a laboratory-scale bioreactor. The procedure was cross-validated with the open-loop digital twin SGR estimator, as well as with the adaptive control of th

SUBMITTER: Urniezius R 

PROVIDER: S-EPMC10781999 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature