The role of PDIA3 in oral squamous cell carcinoma and its value as A diagnostic and prognostic biomarker.

ABSTRACT:

Background

This study aimed to investigate the role of protein disulfide isomerase A3 (PDIA3) in oral squamous cell carcinoma (OSCC) and evaluate its significance as a diagnostic and prognostic biomarker.

Methods

Comprehensive bioinformatics analysis of the OSCC dataset from The Cancer Genome Atlas (TCGA) was performed. PDIA3 was depleted in CAL27 and SCC25 OSCC cells by transfection with PDIA3-specific siRNA oligos. The effects of PDIA3 downregulation on cell viability, apoptosis, and cell migration were evaluated using CCK8, ELISA, and wound healing assays, respectively.

Results

The mRNA and protein expression of PDIA3 was significantly up-regulated in OSCC tissues compared to adjacent normal tissues. Knockdown of PDIA3 led to significantly decreased cell viability,

SUBMITTER: Wang L 

PROVIDER: S-EPMC10782160 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature