The MTCY428.08 gene of Mycobacterium tuberculosis codes for NAD+ synthetase.

ABSTRACT: The product of the MTCY428.08 gene of Mycobacterium tuberculosis shows sequence homology with several NAD+ synthetases. The MTCY428.08 gene was cloned into the expression vectors pGEX-4T-1 and pET-15b. Expression in Escherichia coli led to overproduction of glutathione S-transferase fused and His6-tagged gene products, which were enzymatically assayed for NAD synthetase activity. Our results demonstrate that the MTCY428.08 gene of M. tuberculosis is the structural gene for NAD+ synthetase.

SUBMITTER: Cantoni R 

PROVIDER: S-EPMC107825 | biostudies-literature | 1998 Jun

REPOSITORIES: biostudies-literature