Acute reorganization of postsynaptic GABA_A receptors reveals the functional impact of molecular nanoarchitecture at inhibitory synapses.

ABSTRACT: Neurotransmitter receptors partition into nanometer-scale subdomains within the postsynaptic membrane that are precisely aligned with presynaptic neurotransmitter release sites. While spatial coordination between pre- and postsynaptic elements is observed at both excitatory and inhibitory synapses, the functional significance of this molecular architecture has been challenging to evaluate experimentally. Here we utilized an optogenetic clustering approach to acutely alter the nanoscale organization of the postsynaptic inhibitory scaffold gephyrin while monitoring synaptic function. Gephyrin clustering rapidly enlarged postsynaptic area, laterally displacing GABA_A receptors from their normally precise apposition with presynaptic active zones. Receptor displacement was accompanied

SUBMITTER: Olah SS 

PROVIDER: S-EPMC10782565 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature