Intracellular polyamines regulate redox homeostasis with cAMP-PKA signalling during sexual mating/filamentation and pathogenicity of Sporisorium scitamineum.

ABSTRACT: Sugarcane smut caused by Sporisorium scitamineum seriously impairs sugarcane production and quality. Sexual mating/filamentation is a critical step of S. scitamineum pathogenesis, yet the regulatory mechanisms are not fully understood. In this study, we identified the SsAGA, SsODC, and SsSAMDC genes, which are involved in polyamine biosynthesis in S. scitamineum. Deletion of SsODC led to complete loss of filamentous growth after sexual mating, and deletion of SsAGA or SsSAMDC caused reduced filamentation. Double deletion of SsODC and SsSAMDC resulted in auxotrophy for putrescine (PUT) and spermidine (SPD) when grown on minimal medium (MM), indicating that these two genes encode enzymes that are critical for PUT and SPD biosynthesis. We further showed that low PUT concentrations promoted S.

SUBMITTER: Yin K 

PROVIDER: S-EPMC10782646 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature