Introducing the f₀% method: a reliable and accurate approach for qPCR analysis.

ABSTRACT:

Background

qPCR is a widely used technique in scientific research as a basic tool in gene expression analysis. Classically, the quantitative endpoint of qPCR is the threshold cycle (C_T) that ignores differences in amplification efficiency among many other drawbacks. While other methods have been developed to analyze qPCR results, none has statistically proven to perform better than the C_T method. Therefore, we aimed to develop a new qPCR analysis method that overcomes the limitations of the C_T method. Our f₀% [eff naught percent] method depends on a modified flexible sigmoid function to fit the amplification curve with a linear part to subtract the background noise. Then, the initial fluorescence is estimated and reported as a percentage

SUBMITTER: Gamal M 

PROVIDER: S-EPMC10782791 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature