Expanding the microbiologist toolbox via new far-red-emitting dyes suitable for bacterial imaging.

ABSTRACT:

Importance

By harnessing the versatility of fluorescence microscopy and super-resolution imaging, bacteriologists explore critical aspects of bacterial physiology and resolve bacterial structures sized beyond the light diffraction limit. These techniques are based on fluorophores with profitable photochemical and tagging properties. The paucity of available far-red (FR)-emitting dyes for bacterial imaging strongly limits the multicolor choice of bacteriologists, hindering the possibility of labeling multiple structures in a single experiment. The set of FR fluorophores characterized in this study expands the palette of dyes useful for microbiologists, as they can be used for bacterial LIVE/DEAD staining and for tagging the membranes of viable Escherichia coli and Bacillus

SUBMITTER: Lucidi M 

PROVIDER: S-EPMC10782969 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature