Evidence of the simultaneous replications of active viruses in specimens positive for multiple respiratory viruses.

ABSTRACT:

Importance

Since the pandemic of coronavirus diseases 2019, the use of real-time PCR assay has become widespread among people who were not familiar with it in virus detection. As a result, whether a high real-time PCR value in one time test indicates virus transmissibly became a complicated social problem, regardless of the difference in assays and/or amplification conditions, the time and number of diagnostic test during the time course of infection. In addition, the multiple positives in the test of respiratory viruses further add to the confusion in the interpretation of the infection. To address this issue, we performed virus isolation using pediatric SARI (severe acute respiratory infections) specimens on air-liquid interface culture of human bronchial/tracheal epithelial cell

SUBMITTER: Kawase M 

PROVIDER: S-EPMC10783086 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature