Dataset Information

Secretome of Dental Pulp-Derived Stem Cells Reduces Inflammation and Proliferation of Glioblastoma Cells by Deactivating Mapk-Akt Pathway.

ABSTRACT:

Background

Dental pulp-derived stem cells (DPSC) is a promising therapy as they modulate the immune response, so we evaluated the inhibitory effect of DPSC secretome (DPSC^℗) on the proliferation and inflammation in human glioblastoma (GBM) cells (U-87 MG) and elucidated the concomitant mechanisms involved.

Methods

The U87-MG cells were cultured with DPSC^℗ for 24 h and assessed the expression of inflammatory molecules using quantitative reverse transcription-polymerase chain reaction (qRT-PCR), generation of reactive oxygen species (ROS), and mitochondrial functionality using a seahorse flux analyzer. MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay and cell cycle analysis were performed to evaluate the proliferation and cell cycle. Finally, the protein levels were determined by western blot.

Results

DPSC^℗ reduced the inflammation and proliferation of U-87 MG cells by down-regulating the pro-inflammatory markers and up-regulating anti-inflammatory markers expressions through ROS-mediated signaling. Moreover, DPSC^℗ significantly reduced the mitochondrial membrane potential (MMP) in the cells. The cellular bioenergetics revealed that all the parameters of oxygen consumption rate (OCAR) and the extracellular acidification rate (ECAR) were significantly decreased in the GBM cells after the addition of DPSC^℗. Additionally, DPSC^℗ decreased the GBM cell proliferation by arresting the cell cycle at the G1 phase through activation (phosphorylation) of checkpoint molecule CHK1. Furthermore, mechanistically, we found that the DPSC^℗ impedes the phosphorylation of the mitogen-activated protein kinases (P38 MAPK) and protein kinase B (AKT) pathway.

Conclusion

Our findings lend the first evidence of the inhibitory effects of DPSC^℗ on proliferation and inflammation in GBM cells by altering the P38 MAPK-AKT pathway.

SUBMITTER: Prateeksha P

PROVIDER: S-EPMC10783424 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature

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Secretome of Dental Pulp-Derived Stem Cells Reduces Inflammation and Proliferation of Glioblastoma Cells by Deactivating Mapk-Akt Pathway.

Prateeksha Prateeksha P Howlader Md Sariful Islam MSI Hansda Surajit S Naidu Prathyusha P Das Manjusri M Abo-Aziza Faten F Das Hiranmoy H

Diseases & research 20230719 2

<h4>Background</h4>Dental pulp-derived stem cells (DPSC) is a promising therapy as they modulate the immune response, so we evaluated the inhibitory effect of DPSC secretome (DPSC<sup>℗</sup>) on the proliferation and inflammation in human glioblastoma (GBM) cells (U-87 MG) and elucidated the concomitant mechanisms involved.<h4>Methods</h4>The U87-MG cells were cultured with DPSC<sup>℗</sup> for 24 h and assessed the expression of inflammatory molecules using quantitative reverse transcription-p ...[more]

PMID: 38213319

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Dataset Information

Secretome of Dental Pulp-Derived Stem Cells Reduces Inflammation and Proliferation of Glioblastoma Cells by Deactivating Mapk-Akt Pathway.

Background

Methods

Results

Conclusion

Publications

Secretome of Dental Pulp-Derived Stem Cells Reduces Inflammation and Proliferation of Glioblastoma Cells by Deactivating Mapk-Akt Pathway.

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