A modular dCas9-based recruitment platform for combinatorial epigenome editing.

ABSTRACT: Targeted epigenome editing tools allow precise manipulation and investigation of genome modifications, however they often display high context dependency and variable efficacy between target genes and cell types. While systems that simultaneously recruit multiple distinct 'effector' chromatin regulators can improve efficacy, they generally lack control over effector composition and spatial organisation. To overcome this we have created a modular combinatorial epigenome editing platform, called SSSavi. This system is an interchangeable and reconfigurable docking platform fused to dCas9 that enables simultaneous recruitment of up to four different effectors, allowing precise control of effector composition and spatial ordering. We demonstrate the activity and specificity of the SSSavi system

SUBMITTER: Swain T 

PROVIDER: S-EPMC10783489 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature