Epigenetic profiles guide improved CRISPR/Cas9-mediated gene knockout in human T cells.

ABSTRACT: Genetic modification of specific genes is emerging as a useful tool to enhance the functions of antitumor T cells in adoptive immunotherapy. Current advances in CRISPR/Cas9 technology enable gene knockout during in vitro preparation of infused T-cell products through transient transfection of a Cas9-guide RNA (gRNA) ribonucleoprotein complex. However, selecting optimal gRNAs remains a major challenge for efficient gene ablation. Although multiple in silico tools to predict the targeting efficiency have been developed, their performance has not been validated in cultured human T cells. Here, we explored a strategy to select optimal gRNAs using our pooled data on CRISPR/Cas9-mediated gene knockout in human T cells. The currently available prediction tools alone were insufficient to accuratel

SUBMITTER: Ito Y 

PROVIDER: S-EPMC10783505 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature