TroR is the primary regulator of the iron homeostasis transcription network in the halophilic archaeon Haloferax volcanii.

ABSTRACT: Maintaining the intracellular iron concentration within the homeostatic range is vital to meet cellular metabolic needs and reduce oxidative stress. Previous research revealed that the haloarchaeon Halobacterium salinarum encodes four diphtheria toxin repressor (DtxR) family transcription factors (TFs) that together regulate the iron response through an interconnected transcriptional regulatory network (TRN). However, the conservation of the TRN and the metal specificity of DtxR TFs remained poorly understood. Here we identified and characterized the TRN of Haloferax volcanii for comparison. Genetic analysis demonstrated that Hfx. volcanii relies on three DtxR transcriptional regulators (Idr, SirR, and TroR), with TroR as the primary regulator of iron homeostasis. Bioinformatics and molecu

SUBMITTER: Martinez Pastor M 

PROVIDER: S-EPMC10783522 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature