Ezh2-dependent methylation in oral epithelia promotes secondary palatogenesis.

ABSTRACT:

Background

In addition to genomic risk variants and environmental influences, increasing evidence suggests epigenetic modifications are important for orofacial development and their alterations can contribute to orofacial clefts. Ezh2 encodes a core catalytic component of the Polycomb repressive complex responsible for addition of methyl marks to Histone H3 as a mechanism of repressing target genes. The role of Ezh2 in orofacial clefts remains unknown.

Aims

To investigate the epithelial role of Ezh2-dependent methylation in secondary palatogenesis.

Methods

We used conditional gene-targeting methods to ablate Ezh2 in the surface ectoderm-derived oral epithelium of mouse embryos. We then performed single-cell RNA sequencing combined with immunofluorescence and RT-qPCR t

SUBMITTER: Sun B 

PROVIDER: S-EPMC10784412 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature