Apically localized PANX1 impacts neuroepithelial expansion in human cerebral organoids.

ABSTRACT: Dysfunctional paracrine signaling through Pannexin 1 (PANX1) channels is linked to several adult neurological pathologies and emerging evidence suggests that PANX1 plays an important role in human brain development. It remains unclear how early PANX1 influences brain development, or how loss of PANX1 alters the developing human brain. Using a cerebral organoid model of early human brain development, we find that PANX1 is expressed at all stages of organoid development from neural induction through to neuroepithelial expansion and maturation. Interestingly, PANX1 cellular distribution and subcellular localization changes dramatically throughout cerebral organoid development. During neural induction, PANX1 becomes concentrated at the apical membrane domain of neural rosettes where it co-loca

SUBMITTER: Noort RJ 

PROVIDER: S-EPMC10784521 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature