Measurement of IFN-γ and IL-2 for the assessment of the cellular immunity against SARS-CoV-2.

ABSTRACT: The study of specific T-cell responses against SARS-CoV-2 is important for understanding long-term immunity and infection management. The aim of this study was to assess the dual IFN-γ and IL-2 detection, using a SARS-CoV-2 specific fluorescence ELISPOT, in patients undergoing acute disease, during convalescence, and after vaccination. We also evaluated humoral response and compared with T-cells with the aim of correlating both types of responses, and increase the number of specific response detection. Blood samples were drawn from acute COVID-19 patients and convalescent individuals classified according to disease severity; and from unvaccinated and vaccinated uninfected individuals. IgGs against Spike and nucleocapsid, IgMs against nucleocapsid, and neutralizing antibodies were also anal

SUBMITTER: Safont G 

PROVIDER: S-EPMC10784529 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature