Type 2 inflammation drives an airway basal stem cell program through insulin receptor substrate signaling.

ABSTRACT:

Background

Chronic rhinosinusitis with nasal polyposis (CRSwNP) is a type 2 (T2) inflammatory disease associated with an increased number of airway basal cells (BCs). Recent studies have identified transcriptionally distinct BCs, but the molecular pathways that support or inhibit human BC proliferation and differentiation are largely unknown.

Objective

We sought to determine the role of T2 cytokines in regulating airway BCs.

Methods

Single-cell and bulk RNA sequencing of sinus and lung airway epithelial cells was analyzed. Human sinus BCs were stimulated with IL-4 and IL-13 in the presence and absence of inhibitors of IL-4R signaling. Confocal analysis of human sinus tissue and murine airway was performed. Murine BC subsets were sorted for RNA sequencing and functiona

SUBMITTER: Wang X 

PROVIDER: S-EPMC10784786 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature