Integrating UHPLC-MS/MS quantitative analysis and exogenous purine supplementation to elucidate the antidepressant mechanism of Chaigui granules by regulating purine metabolism.

ABSTRACT: Chaigui granules (CG) are a compound composed of six herbal medicines with significant antidepressant effects. However, the antidepressant mechanism of CG remains unclear. In the present study, we attempted to elucidate the antidepressant mechanism of CG by regulating purine metabolism and purinergic signaling. First, the regulatory effect of CG on purine metabolites in the prefrontal cortex (PFC) of chronic unpredictable mild stress (CUMS) rats was analyzed by ultra high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) targeted quantitative analysis. Meanwhile, purinergic receptors (P2X7 receptor (P2X7R), A₁ receptor (A₁R) and A_2A receptor (A_2AR)) and signaling pathways (nod-like receptor protein 3 (NLRP3) inflammasome pa

SUBMITTER: Chen J 

PROVIDER: S-EPMC10785246 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature