Increased serum extrachromosomal circular DNA SORBS1^circle level is associated with insulin resistance in patients with newly diagnosed type 2 diabetes mellitus.

ABSTRACT:

Background

Extrachromosomal circular DNAs (eccDNAs) exist in human blood and somatic cells, and are essential for oncogene plasticity and drug resistance. However, the presence and impact of eccDNAs in type 2 diabetes mellitus (T2DM) remains inadequately understood.

Methods

We purified and sequenced the serum eccDNAs obtained from newly diagnosed T2DM patients and normal control (NC) subjects using Circle-sequencing. We validated the level of a novel circulating eccDNA named sorbin and SH3-domain- containing-1^{circle97206791-97208025} (SORBS1^circle) in 106 newly diagnosed T2DM patients. The relationship between eccDNA SORBS1^circle and clinical data was analyzed. Furthermore, we explored the source and expression level of eccDNA SORBS1^circl

SUBMITTER: Kong X 

PROVIDER: S-EPMC10785328 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature