A cautionary tale of low-pass sequencing and imputation with respect to haplotype accuracy.

ABSTRACT:

Background

Low-pass whole-genome sequencing and imputation offer significant cost savings, enabling substantial increases in sample size and statistical power. This approach is particularly promising in livestock breeding, providing an affordable means of screening individuals for deleterious alleles or calculating genomic breeding values. Consequently, it may also be of value in companion animal genomics to support pedigree breeding. We sought to evaluate in dogs the impact of low coverage sequencing and reference-guided imputation on genotype concordance and association analyses.

Results

DNA isolated from saliva of 30 Labrador retrievers was sequenced at low (0.9X and 3.8X) and high (43.5X) coverage, and down-sampled from 43.5X to 9.6X and 17.4X. Genotype imputation was pe

SUBMITTER: Wragg D 

PROVIDER: S-EPMC10785484 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature