Comparison of the extracellular vesicle proteome between glaucoma and non-glaucoma trabecular meshwork cells.

ABSTRACT:

Introduction

Extracellular matrix (ECM) materials accumulate in the trabecular meshwork (TM) tissue of patients with glaucoma, which is associated with a decrease in aqueous humor outflow and therefore an increase in intraocular pressure. To explore a potential mechanism for ECM regulation in the TM, we purified extracellular vesicles (EVs) from conditioned media of differentiated TM cells in culture isolated from non-glaucomatous and glaucomatous human donor eyes.

Methods

EVs were purified using the double cushion ultracentrifugation gradient method. Fractions containing EV markers CD9 and TSG101 were analyzed using nanoparticle tracking analysis to determine their size and concentration. We then determined their proteomic cargo by mass spectrometry and compared protein pro

SUBMITTER: McDonnell FS 

PROVIDER: S-EPMC10785745 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature