In Vivo Calcium Imaging of Neuronal Ensembles in Networks of Primary Sensory Neurons in Intact Dorsal Root Ganglia.

ABSTRACT: Ca²⁺ imaging can be used as a proxy for cellular activity, including action potentials and various signaling mechanisms involving Ca²⁺ entry into the cytoplasm or the release of intracellular Ca²⁺ stores. Pirt-GCaMP3-based Ca²⁺ imaging of primary sensory neurons of the dorsal root ganglion (DRG) in mice offers the advantage of simultaneous measurement of a large number of cells. Up to 1,800 neurons can be monitored, allowing neuronal networks and somatosensory processes to be studied as an ensemble in their normal physiological context at a populational level in vivo. The large number of neurons monitored allows the detection of activity patterns that would be challenging to detect using other methods. Stimuli can be applied to the mouse hindpaw,

SUBMITTER: Shannonhouse J 

PROVIDER: S-EPMC10785773 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature