Cellular Visualization of G-Quadruplex RNA via Fluorescence- Lifetime Imaging Microscopy.

ABSTRACT: Over the past decade, appreciation of the roles of G-quadruplex (G4) structures in cellular regulation and maintenance has rapidly grown, making the establishment of robust methods to visualize G4s increasingly important. Fluorescent probes are commonly used for G4 detection in vitro; however, achieving sufficient selectivity to detect G4s in a dense and structurally diverse cellular environment is challenging. The use of fluorescent probes for G4 detection is further complicated by variations of probe uptake into cells, which may affect fluorescence intensity independently of G4 abundance. In this work, we report an alternative small-molecule approach to visualize G4s that does not rely on fluorescence intensity switch-on and, thus, does not require the use of molecules with exclusive G4

SUBMITTER: Robinson J 

PROVIDER: S-EPMC10786036 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature