Engineering a Plant Polyketide Synthase for the Biosynthesis of Methylated Flavonoids.

ABSTRACT: Homoeriodictyol and hesperetin are naturally occurring O-methylated flavonoids with many health-promoting properties. They are produced in plants in low abundance and as complex mixtures of similar compounds that are difficult to separate. Synthetic biology offers the opportunity to produce various flavonoids in a targeted, bottom-up approach in engineered microbes with high product titers. However, the production of O-methylated flavonoids is currently still highly inefficient. In this study, we investigated and engineered a combination of enzymes that had previously been shown to support homoeriodictyol and hesperetin production in Escherichia coli from fed O-methylated hydroxycinnamic acids. We determined the crystal structures of the enzyme catalyzing the first committed step of

SUBMITTER: Peng B 

PROVIDER: S-EPMC10786038 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature