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Complex optically computed phase microscopy for label-free sub-cellular imaging.

ABSTRACT: In this study, we investigated a complex optically computed phase microscopy (complex-OCPM) technology. Based on a low coherence interferometer and an innovative optical computation approach, the complex-OCPM imaging system achieves depth resolved quantitative phase measurement. Particularly, the complex-OCPM imaging system directly measures the complex amplitude of the optical field emerging from the sample, extracts the phase as the argument of a complex signal, and achieves a high spatial resolution in phase imaging. We evaluated the performance of complex-OCPM imaging using resolution targets and live cells. Our results show that the complex-OCPM system achieves quantitative phase imaging with sub-cellular resolution on label-free cells.

SUBMITTER: Liu X

PROVIDER: S-EPMC10786169 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature

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Complex optically computed phase microscopy for label-free sub-cellular imaging.

Liu Xuan X Liu Yuwei Y Yu Shupei S Zhang Yuanwei Y

Optics continuum 20230327 4

In this study, we investigated a complex optically computed phase microscopy (complex-OCPM) technology. Based on a low coherence interferometer and an innovative optical computation approach, the complex-OCPM imaging system achieves depth resolved quantitative phase measurement. Particularly, the complex-OCPM imaging system directly measures the complex amplitude of the optical field emerging from the sample, extracts the phase as the argument of a complex signal, and achieves a high spatial res ...[more]

PMID: 38223255

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