Multiscale spatial mapping of cell populations across anatomical sites in healthy human skin and basal cell carcinoma.

ABSTRACT: Our understanding of how human skin cells differ according to anatomical site and tumour formation is limited. To address this, we have created a multiscale spatial atlas of healthy skin and basal cell carcinoma (BCC), incorporating in vivo optical coherence tomography, single-cell RNA sequencing, spatial global transcriptional profiling, and in situ sequencing. Computational spatial deconvolution and projection revealed the localisation of distinct cell populations to specific tissue contexts. Although cell populations were conserved between healthy anatomical sites and in BCC, mesenchymal cell populations including fibroblasts and pericytes retained signatures of developmental origin. Spatial profiling and in silico lineage tracing support a hair follicle origin for BCC and demonstrate t

SUBMITTER: Ganier C 

PROVIDER: S-EPMC10786309 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature