The tensile and viscoelastic properties of aortic valve leaflets treated with a hyaluronidase gradient.

ABSTRACT:

Purpose

When diseased, aortic valves are typically replaced with bioprosthetic heart valves (BPHVs), porcine valves or bovine pericardium that are fixed in glutaraldehyde. These replacements fail within 10-15 years due to calcification and fatigue, and their failure coincides with a loss of glycosaminoglycans (GAGs). This study investigates this relationship between GAG concentration and the tensile and viscoelastic properties of aortic valve leaflets.

Methods

Aortic valve leaflets were dissected from porcine hearts and digested in hyaluronidase in concentrations ranging from 0-5 U/mL for 0-24 hours, yielding a spectrum of GAG concentrations that was measured using the uronic acid assay and confirmed by Alcian Blue staining. Digested leaflets with varying GAG concentrations

SUBMITTER: Tseng H 

PROVIDER: S-EPMC10786346 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature